Dynamic Light Scattering Instrument: Litesizer DLS
Litesizer DLS delivers best-in-class particle sizing with automatic angle selection to prevent errors, while MAPS technology yields the highest-possible peak resolution.
Manufacturer: Anton Paar — Austria
Overview
Litesizer DLS delivers best-in-class particle sizing with automatic angle selection to prevent errors, while MAPS technology yields the highest-possible peak resolution. Continuous transmittance monitoring detects sedimentation and agglomeration during measurement, enhancing measurement reliability. Unique to our dynamic light scattering instrument, cmPALS and the Omega Cuvette improve zeta potential accuracy and repeatability by addressing aging effects and minimizing electrical gradient. Our dynamic light scattering instruments also offer fluorescence and polarization filters, not only for particle size measurements, but also for particle concentration in both MAPS three-angle and single-angle modes.
Three Angles, Real-Time Monitoring for Better Sizing
Litesizer DLS offers the choice between three different detection angles to measure a wide array of samples, while eliminating errors with automatic angle selection. The Multi-Angle Particle Sizing (MAPS) measurement mode delivers exceptional resolution, supporting advanced analytical precision. Unique to our DLS particle size analyzers, continuous transmittance monitoring provides live feedback on sample behavior throughout measurement, giving users instant information on sedimentation or agglomeration. This real-time insight enhances measurement quality and reliability, making our dynamic light scattering instrument the optimal choice for accurate and dependable particle analysis.
Market-Leading Zeta Potential Analysis
Our dynamic light scattering instrument's patented cmPALS technique is specially designed to eliminate reproducibility uncertainties caused by aging effects. Additionally, the Omega Cuvette's innovative design minimizes the gradient in the electrical field, further enhancing reproducibility (±3 %). With an industry-leading particle size range for zeta potential measurement, from 1.3 nm to 100 µm, our dynamic light scattering instrument offers the broadest capability available, ensuring precise and consistent results across a vast array of applications.
Kalliope: The Benchmark Software for Particle Sizing
The Kalliope software requires very little user training. Measurements with the dynamic light scattering instrument can be performed with only three clicks. The one-of-a-kind one-page layout provides an instant overview of input parameters, measurement signals, and results, presenting everything you need at a glance. Quickly access measurement results with predefined standard reports and organized result summaries. For more detailed analysis, use the customizable report templates, Excel export options, and advanced analysis functions. Kalliope is also fully compliant with 21 CFR Part 11, ensuring robust data integrity and regulatory adherence.
All the Measuring Modes You Need
Enhance your versatility with Litesizer DLS 701's particle concentration measurement mode, enabling calibration-free concentration analysis for up to three distinct size populations within a single sample. This mode supports both monodisperse and polydisperse samples across a wide concentration range, whether you're using single-angle DLS or MAPS. You can additionally measure the refractive index of solvents at the exact wavelength and temperature of your measurements (European Patent 3 023 770), ensuring maximum accuracy for particle size and zeta potential results. Litesizer DLS 701 and DLS 501 provide precise refractive index measurements within ±0.5 %. Our dynamic light scattering instrument also offers molecular mass measurement.
Most Capable Filtering Options Available
Litesizer DLS 701 and 501 are the only dynamic light scattering instruments on the market that offer users the choice between fluorescence or polarization filters, which can be applied in all three angle directions. This enables particle concentration analysis as well as MAPS three-angle or selected single-angle analysis with applied filters – permitting types of analysis not possible with comparable instruments (e.g., concentration determination of quantum dots).
10-Year Laser Warranty: Confidence, for a Decade
Each Litesizer DLS is built around a high-performance laser system engineered for long-term stability and reliable operation. We make sure our instruments' core optical performance supports precise, reproducible DLS-based particle size measurements for many years, regardless of application demands. That's why we offer a 10-year warranty on the laser – a commitment that goes far beyond industry standards. We'll win your confidence, for a decade.
Models
Dynamic Light Scattering Instrument: Litesizer DLS is available in 4 models: Litesizer DLS 701, Litesizer DLS 501, Litesizer DLS 301, Litesizer DLS 101.
Litesizer DLS 701
Particle sizing from 0.3 nm to 15 µm.
Litesizer DLS 501
Particle sizing from 0.3 nm to 15 µm.
Litesizer DLS 301
Particle sizing from 0.3 nm to 15 µm.
Litesizer DLS 101
Particle sizing from 0.3 nm to 10 µm.
Specifications
| Specification | Litesizer DLS 701 | Litesizer DLS 501 | Litesizer DLS 301 | Litesizer DLS 101 |
|---|---|---|---|---|
| Particle Size Specifications | ||||
| Measurement Principle | Dynamic Light Scattering (DLS) | |||
| Measuring Range | 0.3 nm to 15 μm* (particle diameter) | 0.3 nm to 15 μm* (particle diameter) | 0.3 nm to 15 μm* (particle diameter) | 0.3 nm to 10 μm* (particle diameter) |
| Measurement Angles | 15°, 90°, 175°, Multi-angle particle sizing (MAPS) | 15°, 90°, 175° | 90° | 175° |
| Min. Concentration | 0.1 mg/mL (lysozyme) lower than 0.00001 % (0.1 ppm, Latex 100 nm) | 0.1 mg/mL (lysozyme) lower than 0.00001 % (0.1 ppm, Latex 100 nm) | 1 mg/mL (lysozyme) | 0.1 mg/mL (lysozyme) |
| Max. Concentration | 50 % w/v (sample-dependent) | 50 % w/v (sample-dependent) | 40 % w/v (sample-dependent) | 50 % w/v (sample-dependent) |
| Min. Sample Volume | 1.5 µL | 1.5 µL | 1.5 µL | 12 µL |
| Accuracy | Better than ±2 % on NIST traceable standards | |||
| Repeatability | Better than ±2 % on NIST traceable standards | |||
| Particle Concentration Specifications | ||||
| Analysis Model | Mie Theory | - | - | - |
| Measuring Range | 108 - 1013 particles/mL (sample dependent) | - | - | - |
| Size Limit | 1 µm | - | - | - |
| Measurement Angles | 15°, 90°, 175°, Multi-angle particle sizing (MAPS) | - | - | - |
| Min. Sample Volume | 12 µL | - | - | - |
| Accuracy | ± 10 % (sample dependent) | - | - | - |
| Repeatability | ± 5 % (sample dependent) | - | - | - |
| Zeta Potential Specifications | ||||
| Measuring Principle | Electrophoretic Light Scattering (ELS) / cmPALS | Electrophoretic Light Scattering (ELS) / cmPALS | Electrophoretic Light Scattering (ELS) / cmPALS | - |
| Measuring Range | >= ±1000 mV | >= ±1000 mV | >= ±1000 mV | - |
| Mobility Range | 10⁻¹¹ m²/V.s to 2 x 10⁻⁷ m²/V.s | 10⁻¹¹ m²/V.s to 2 x 10⁻⁷ m²/V.s | 10⁻¹¹ m²/V.s to 2 x 10⁻⁷ m²/V.s | - |
| Size Range | 1.3 nm to 100 µm (diameter) | 1.3 nm to 100 µm (diameter) | 1.3 nm to 100 µm (diameter) | - |
| Min. Sample Concentration | 0.1 mg/mL (lysozyme) | 0.1 mg/mL (lysozyme) | 0.1 mg/mL (lysozyme) | - |
| Max. Sample Concentration | 70 %w/v (sample-dependent) | 70 %w/v (sample-dependent) | 70 %w/v (sample-dependent) | - |
| Max. Sample Conductivity | 200 mS/cm | 200 mS/cm | 200 mS/cm | - |
| Min. Sample Volume | 50 µL (sample-viscosity-dependent) | 50 µL (sample-viscosity-dependent) | 50 µL (sample-viscosity-dependent) | - |
| Accuracy | Better than ±10 % | Better than ±10 % | Better than ±10 % | - |
| Repeatability | ±3 % | ±3 % | ±3 % | - |
| Molecular Mass Specifications | ||||
| Measuring Principle | Static Light Scattering (SLS) | Static Light Scattering (SLS) | Static Light Scattering (SLS) | - |
| Measuring Range (Mass) | 300 Da to 20 MDa | 300 Da to 20 MDa | 300 Da to 20 MDa | - |
| Measuring Range (Particle Size) | Up to 40 nm (diameter) | Up to 40 nm (diameter) | Up to 40 nm (diameter) | - |
| Measurement Angle | 90° | 90° | 90° | - |
| Min. Sample Concentration | 0.1 mg/mL (lysozyme) | 0.1 mg/mL (lysozyme) | 0.1 mg/mL (lysozyme) | - |
| Accuracy | ±10 % | ±10 % | ±10 % | - |
| Repeatability | ±5 % | ±5 % | ±5 % | - |
| Transmittance Specifications | ||||
| Measuring Range | 0 % to 100 % | |||
| Min. Sample Volume | 15 µL | |||
| Accuracy | Better than ±1 % | |||
| Refractive Index Specifications | ||||
| Measuring Range | 1.28 to 1.50 | 1.28 to 1.50 | - | - |
| Min. Sample Volume | 1 mL | 1 mL | - | - |
| Accuracy | ±0.5 % | ±0.5 % | - | - |
| General Specifications | ||||
| Light Source | Semiconductor laser diode / 40 mW, 658 nm | |||
| Optical Filters (Fluorescence, Vertical and Horizontal Polarization) | forward, side and backscattering | forward, side and backscattering | forward, side and backscattering | - |
| Laser Warm-Up Time | 6 min | |||
| Temperature Control Range | 0 °C to 120 °C | |||
| Ambient Operating Temperature | 10 °C to 35 °C | |||
| Humidity | Up to 80 % non-condensing | |||
| Dimensions (W, D, H) | 450 mm, 505 mm, 135 mm | |||
| Weight | Approx. 18 kg (40 lb) | Approx. 18 kg (40 lb) | Approx. 18 kg (40 lb) | Approx. 16.3 kg (36 lb) |