InSight® X3+™/X3™

The InSight X3+ and InSight X3 are built on Spectra-Physics' industry-leading InSight platform, designed for maximum performance and reliability in multiphoton microscopy.

Spectra-Physics InSight® X3+™/X3™ — Ultrafast Laser Systems

Manufacturer: Spectra-Physics — USA

Overview

The InSight X3+ and InSight X3 are built on Spectra-Physics' industry-leading InSight platform, designed for maximum performance and reliability in multiphoton microscopy. Featuring a gap-free tuning range from 680 nm to 1300 nm, InSight is a hands-free, fully computer-controlled light source with the largest installed base in the market, generating more than 1,000 peer-reviewed publications. Performance is optimized in the 900-1100 nm range to support common green and red fluorescent proteins, while the InSight X3+ delivers higher power across the tuning range for brighter signals and increased imaging depths.

Ultra-Wide Tuning Range

The InSight platform is designed for advanced multiphoton microscopy in neuroscience, immunology, and other biological sciences, featuring a gap-free 680 nm to 1300 nm tuning range that nearly doubles the accessible wavelengths compared to traditional Ti:Sapphire lasers. Performance is optimized in the 900-1100 nm range to support common green and red fluorescent proteins including GFP, RFP, GCaMP, jRGECO, and mCherry.

Exceptional Performance

Both lasers can be equipped with a fixed 1045 nm dual beam option to support multimodal imaging, with the tunable and fixed outputs synchronized to enable advanced techniques such as CARS and SRS. The InSight also supports simultaneous multi-wavelength excitation of fluorophores and second and third harmonic generation microscopies.

Common Specifications

SpecificationInSight® X3+™/X3™
Repetition Rate80 MHz ±0.5
Spatial ModeTEM00, M² <1.2
Beam Diameter (1/e²)1.1 ±0.2 mm
Beam Divergence, full angle<1.5 mrad
Beam Roundness0.8-1.2
Beam Astigmatism<20%
Beam Pointing Stability<350 µrad
Tuning Speed>50 nm/sec
Stability<±1%
Polarization>500:1 horizontal

Model Comparison

SpecificationInSight X3+InSight X3
Tuning Range (Main Output)680 nm-1300 nm680 nm-1300 nm
Average Power at 900 nm (Main Output)>3.0 W>2.0 W
Pulse Width (Main Output)<120 fs<120 fs
Dual Output Power>3.5 W>3.5 W

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