InSight® X3+™/X3™
The InSight X3+ and InSight X3 are built on Spectra-Physics' industry-leading InSight platform, designed for maximum performance and reliability in multiphoton microscopy.

Manufacturer: Spectra-Physics — USA
Overview
The InSight X3+ and InSight X3 are built on Spectra-Physics' industry-leading InSight platform, designed for maximum performance and reliability in multiphoton microscopy. Featuring a gap-free tuning range from 680 nm to 1300 nm, InSight is a hands-free, fully computer-controlled light source with the largest installed base in the market, generating more than 1,000 peer-reviewed publications. Performance is optimized in the 900-1100 nm range to support common green and red fluorescent proteins, while the InSight X3+ delivers higher power across the tuning range for brighter signals and increased imaging depths.
Ultra-Wide Tuning Range
The InSight platform is designed for advanced multiphoton microscopy in neuroscience, immunology, and other biological sciences, featuring a gap-free 680 nm to 1300 nm tuning range that nearly doubles the accessible wavelengths compared to traditional Ti:Sapphire lasers. Performance is optimized in the 900-1100 nm range to support common green and red fluorescent proteins including GFP, RFP, GCaMP, jRGECO, and mCherry.
Exceptional Performance
Both lasers can be equipped with a fixed 1045 nm dual beam option to support multimodal imaging, with the tunable and fixed outputs synchronized to enable advanced techniques such as CARS and SRS. The InSight also supports simultaneous multi-wavelength excitation of fluorophores and second and third harmonic generation microscopies.
Common Specifications
| Specification | InSight® X3+™/X3™ |
|---|---|
| Repetition Rate | 80 MHz ±0.5 |
| Spatial Mode | TEM00, M² <1.2 |
| Beam Diameter (1/e²) | 1.1 ±0.2 mm |
| Beam Divergence, full angle | <1.5 mrad |
| Beam Roundness | 0.8-1.2 |
| Beam Astigmatism | <20% |
| Beam Pointing Stability | <350 µrad |
| Tuning Speed | >50 nm/sec |
| Stability | <±1% |
| Polarization | >500:1 horizontal |
Model Comparison
| Specification | InSight X3+ | InSight X3 |
|---|---|---|
| Tuning Range (Main Output) | 680 nm-1300 nm | 680 nm-1300 nm |
| Average Power at 900 nm (Main Output) | >3.0 W | >2.0 W |
| Pulse Width (Main Output) | <120 fs | <120 fs |
| Dual Output Power | >3.5 W | >3.5 W |
