Delphi-X Inverso Fluorescence Series

Overview

The Delphi-X Inverso for fluorescence is an inverted microscope for the professional who needs to observe tissues or cells stained with fluorescence dyes. Super contrast semi-apochromatic Fluarex objectives with collar adjustment ring assure perfect observation of cells or tissues in 1.2 mm thick Petri dishes or flasks.

Key Highlights

• Life science applications
• Observation of fluorescence in tissues and cells
• Brightfield, phase contrast fluorescence observations
• DIC (differential interference contrast)
• Long working distance IOS plan semi-apochromatic Fluarex achromatic objectives
• Revolving sextuple nosepiece
• 5 W LED or 100 W halogen transmitted illumination
• 100 W HBO mercury vapor fluorescence illumination
• Backwards tilting condenser arm
• Easy placement of large objects

Specifications

EYEPIECES

Pair of DIN HWF plan 10x/22 mm eyepieces with diopter adjustment.

HEAD

Trinocular 45° inclined tubes. Interpupillary distance of 47 to 78 mm. Trinocular head with 100-0, 80/20 and 0/100 positions.

NOSEPIECE

Revolving sextuple nosepiece on ball-bearings. The nosepiece can be equipped with DIC prisms for the 10x/20x and 40x objectives.

STAGE

Stage 340 x 230 mm, equipped with a coaxial mechanical 130 x 85 mm X-Y stage. Supplied with:

• Metal insert for Ø 38 mm cell culture dish
• Metal insert for Ø 54 mm cell culture dish or 76 x 26 mm slides
• Metal insert for Multiwell plates (127.76 x 85.48 mm)
• Metal insert for Teraski plates (83.3 x 58.0 mm)
• Optional universal cell culture holder

CONDENSER

Rotating N.A. 0.55 condenser with iris diaphragm. Working distance 26 mm. Supplied with phase contrast annuli for 10x/20x, 40x and 60x objectives and the DIC prisms for the 10x/20x/40x and 60x objectives. Three filter holders allow the insertion of Ø 38 mm filters in the optical path. The arm with condenser can be tilted backwards for convenient access to the stage. This allows easy placement of large objects like flasks and Petri dishes.

LENS CHANGER

A lens changer with a 1.5x lens allows intermediary magnifications of 1.5 times the standard objective magnifications. Switching from 20x to 30x or 40 to 60 and from 60x to 90x magnification can be done without the need to re-focus on the sample.

FLUORESCENCE ATTACHMENT

The Delphi-X Inverso for fluorescence microscopy is supplied with a 6-position fluorescence filter set wheel and comes with two fluorescence filter sets for blue and green excitation (DI.9745 and DI.9746). Other filter sets are optional.

DI.9745 Blue excitation, EX 460-495 DM 505 EM 510 long pass.

DI.9746 Green excitation, EX 510-550 DM 570 EM 575 long pass.

DI.9747 Violet excitation, EX 400-410 DM 455 EM 460 Long pass.

DI.9748 Ultra violet excitation, EX 330-385 DM 410 EM 420 Long pass.

DI.9750.TRITC Green excitation, EX 528-553 DM 565 EM 578-633 band pass.

DI.9750.FITC Blue excitation, EX 460-495 DM 505 EM 510-550 band pass.

DI.9750.DAPI UV excitation, EX 360-390 DM 415 EM 435-485 band pass.

DI.9750.R Red excitation, EX 620-650 DM 660 EM 670-750 band pass.

FOCUSING ADJUSTMENT

Coaxial 10 mm coarse and fine adjustments, 1 μm precision and with friction adjustment.

ILLUMINATION

Fluorescence illumination with a 100 W mercury vapor lamp with external 100-240 V power supply. Transmitted 5 W LED or 100 W halogen illumination and internal power supply. Fuse holder 3.15 A / 250 V.

PACKAGE CONTENT

Supplied with metal insert for Ø 38 mm cell culture dish and a metal insert for Ø 54 mm cell culture dish with 76 x 26 mm slides, metal insert for Multiwell plates, metal insert for Teraski plates, blue and green interference Ø 45 mm filters, power cord, dust cover, green filter and alignment telescope and user manual.

Delphi-X Inverso fluorescence